Abstract: The train operation adjustment rules and strategies under other organizational modes are sorted in detail, and the concept of smart agent is introduced. Referring to the movement effect of "sardine herd", the running time interval between adjacent two trains is divided into three thresholds, i.e., exclusion interval, cooperative interval and attraction interval, and the cooperative movement relationship between trains is established. Self-organization operation adjustment algorithm in case of train delay is realized with simulation, which demonstrates the effectiveness and applicability of the self-organization adjustment mechanism.