Abstract: Trans-regional urban rail transit promotes the integrated development of urban agglomeration transportation. Current research status of trans-regional urban rail transit lines is analyzed, as well as the development status of domestic and foreign associated lines and operation management experience. Through analysis of line connection form and operation organization, operation management framework, operation management points, trans-regional line operation and management strategy in China is proposed. Taking Guangzhou and Foshan urban rail transit network interoperation as a study case, operation and management mode, communication and coordination mechanism, dispatch command, ticket sorting and other related practices of trans-regional urban rail transit lines are analyzed, and suggestions for development are put forward.