Abstract: Based on Nanjing metro Line 5 that crosses under Line 3, a 3D finite element model of fourhole overlapping tunnel is established by using ABAQUS software, to calculate the dynamic stress of soil under the action of train dynamic load, and predict the long-term settlement of the overlapping tunnels with empirical formula. The results show that the maximum settlement of overlapping tunnels is 16.3 mm, the radius of curvature is 13 589 m, relative curvature is 1/1 100, all exceed the specifications, and settlement control measures must be taken. It is proposed to adopt back wall grouting reinforcement measures during the construction of Line 5 tunnels. The result shows that the maximum settlement is reduced to 10.4 mm, the radius of curvature increased to 18 750 m, and the relative deflection reduced to 1/2 575 by taking grouting reinforcement measurement, all meet the specifications. Therefore, the strata reinforcement measures can control the settlement in overlapping areas effectively and ensure the long-term service of tunnel structure.