Abstract: For the operational safety of the main structure of Wuhan Fuxing Road underground station, the structural mechanical response with different boundary conditions and unfavorable loading conditions during the operational period is studied by means of refined numerical analysis using a three-dimensional full-size model. The study shows that: improper foundation reinforcement treatment will induce excessive local settlement of floor slabs and longitudinal beams, and the maximum differential settlement between columns increases by 43%; the top slab settlement increases by 33% in condition of ground surface overload such as precipitation and construction of high-rise buildings, and the total settlement reaches 51.9 mm, while the ground surface overload should not exceed designed overload value; the locally developed cavities have insignificant effect on the force and deformation force on the main structure of the station, thus it can be overlooked. Combining the results of the structural force performance analysis at design stage and under extreme working conditions, the parts of structural force weakness, significant deformation zones and monitoring indexes that require emphatical attention during the operational period are identified, and the control and early warning standards for each index are formulated.