Abstract: Vibration and shock of urban rail transit trains during operation are inevitable, which cause damage or even failure to train on-board signaling equipment. Thus, it is necessary to consider the application of vibration isolation scheme of on-board signaling equipment. When designing the vibration isolation scheme, it is necessary to consider the structural form, mechanical features, installation method and location of on-board signaling equipment. Through application of vibration isolation scheme on urban rail transit train on-board signaling equipment, the expected and actual effects are comparatively analyzed, it shows that the vibration isolation scheme can effectively isolate or absorb the vibration and shock generated during vehicle operation and ensuve reliable operation of the signaling equipment within its service life.