Abstract: To compare the application effect of resonant floating rail fastener and single toe spring fastener in track vibration reduction, finite element analysis is adopted to compare the merits and demerits of their dumping effect. Based on field measured data, in the case of Guangzhou Metro Line 5 interval up and down lines between Yuzhu Station and Dashadi Station, the track dynamic deformation and vibration condition at the same measuring point before and after replacing resonant floating rail fastener with single toe spring fastener is analyzed. In addition, test analysis is carried out on sensitive building vibration and the change level of secondary radiation noise level. Research results show that resonant floating rail fastener has a significant effect on reducing wheel-rail vibration, and the effect on reducing secondary structural noise in surrounding environment is also better than that of single toe spring fastener.