Abstract: In view of the current epidemic prevention and control disinfection requirements for public transportation system in China, the application potential and prospect of human-machine co-existence disinfection is analyzed from aspects including Far UV-C (ultraviolet-C) disinfection principle and effect, Far UV-C disinfection effectiveness research, Far UV-C eye and skin safety nontoxicity principle, Far UV-C human body safety research. The Far UV-C technology disinfection application status is briefly introduced from technical aspects such as safety threshold and O3 production amount control. The application prospect of Far UV-C technology disinfection is analyzed in terms of Far UV-C disinfection light-surface disinfection, Far UV-C disinfection passage-surface disinfection, and Far UV-C disinfection filter-air dissolver disinfection, and the potential problems of the technology in disinfection application are given.