Abstract: Objective: Based on the requirements of Hefei Rail Transit Line 4 and Line 6 signaling system splitting engineering, a targeted study on the splitting plan is necessary. Method: The demands for Line 4 and Line 6 signaling system splitting are analyzed, as well as the interference issue of LTE (longterm evolution) system between the two lines during sameplatform interchange at Beiyanhu Station after splitting. The splitting plans for Line 4 and Line 6 signaling system are introduced, including segmented wayside design plan, splitting plan for Beiyinhu station as the splitting station, splitting design plan for Kexuecheng Depot, and splitting design plan for DCS (data communication system) subsystem. The antiinterference design scheme for LTE systems of the two lines at Beiyanhu Station is also presented. Result & Conclusion: Engineering practice demonstrates that the key to Line 4 and Line 6 signaling system splitting lies in early preservation and splitting plan design. By providing complete preservation for splitting conditions and making full use of existing equipment, the amount of engineering work required for splitting implementation can be reduced, and potential impacts on existing line operation during testing and commissioning can be avoided.